Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. 1999.

نویسندگان

  • Yukio Arita
  • Shinji Kihara
  • Noriyuki Ouchi
  • Masahiko Takahashi
  • Kazuhisa Maeda
  • Jun-ichiro Miyagawa
  • Kikuko Hotta
  • Iichiro Shimomura
  • Tadashi Nakamura
  • Koji Miyaoka
  • Hiroshi Kuriyama
  • Makoto Nishida
  • Shizuya Yamashita
  • Kosaku Okubo
  • Kenji Matsubara
  • Masahiro Muraguchi
  • Yasuichi Ohmoto
  • Tohru Funahashi
  • Yuji Matsuzawa
چکیده

We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K., et al., Biochem. Biophys. Res. Commun. 221, 286-289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans.

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عنوان ژورنال:
  • Biochemical and biophysical research communications

دوره 425 3  شماره 

صفحات  -

تاریخ انتشار 1999